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All physiological stimuli result in changes in the level of activity of the autonomic nervous system. A method of monitoring the over-all activity of the adrenergic nerves and adrenal medullae would greatly simplify the interpretation of many physiological events. The present work began with the assumption that blood levels of norepinephrine (NE) and epinephrine (E) might reflect adrenergic activity. Therefore, our initial objective was to modify one of the existing chemical methods for the measurement of NE and E so that it would be suitable for our purposes. However, we became impressed with the controversy in the literature over the chemical identification of plasma NE and E and, as a result, adopted the dual criteria that plasma material must have not only the chemical properties of NE and E but must be removed by the tissues like NE and E to justify identification as these substances. The trihydroxyindole (THI) method of Lund (1-4) and the ethylenediamine (EDA) method of Weil-Malherbe and Bone (5-7) are the two methods currently considered suitable for analysis of plasma NE and E. Neither method is specific. In this respect, the EDAmethod is inferior to the THI method (6, 8, 9), a fact which is emphasized by the false high readings of the former in uremia (10). However, the THI method may measure dopamine (3, 4), dihydroxyphenylalanine (2-4), isopropylnorepinephrine (2-4, 9), serotonin (2) or aureomycin (11), depending on the

ARTERIOVENOUS DIFFERENCES OF THE NOREPINEPRINE-LIKE MATERIAL FROM NORMAL PLASMA AND INFUSED NOREPINEPHRINE*