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There have been relatively few attempts to study the dynamic aspects of nitrogen metabolism from the standpoint of the intact overall organism. The complexity of the problem precludes an exact mathematical description at present, but models which purport to enable one to compute values for nitrogeneous metabolic pool size and rate of incorporation of nitrogen into the human have been devised by San Pietro and Rittenberg (1) and Olesen, Heilskov and Schoenheyder (2). The approach of San Pietro and Rittenberg was to devise a model on biologic grounds and describe the model by equations, whereas that of Olesen and co-workers was to derive a model from experimental data which could be described by certain equations. The latter, by extending the length of time of the experiment, changed the nature of some of the necessary assumptions of the San Pietro-Rittenberg type model. While there are certain advantages and disadvantages inherent in both types of approach, it would appear that the San Pietro-Rittenberg approach is as satisfactory as any yet available. The purpose of this study of the San PietroRittenberg model was to determine: 1) the reproducibility of calculated values for nitrogenous metabolic pool size (P), rate of nitrogen incorporation into the protein of the organism (S), and rate constant for metabolic pool turnover (B) in one individual under the same experimental conditions, and 2) the effects of variation of caloric and protein content of the diet on the calculated values. Further mathematical manipulations of the original equations have led to an expression which allows the computation of B at any time, thus providing further insight into the model. METHODS AND MATERIALS

STUDIES OF THE EFFECT OF DIETARY PROTEIN AND CALORIC LEVELS ON THE KINETICS OF NITROGEN METABOLISM USING N15 L-ASPARTIC ACID