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The in vitro influence of glucose, fructose, and insulin on human leukocytes of healthy and diabetic subjects was measured as a part of a study of the metabolic behavior of these cells, and an attempt was made to correlate metabolic alterations in leukocytes with resistance and susceptibility to infectious diseases. Previous workers have shown that leukocytes from alloxan diabetic rabbits are able to phagocytize but not destroy bacteria (1). Dubos has shown that lactate in the presence of low oxygen tension is bactericidal for M. tuberculosis (2). Human leukocytes produce lactic acid in the presence of oxygen, and the quantity is not changed markedly by decreased oxygen tension (3, 4). It has also been shown that these cells produced more lactate from glucose than from fructose, and that when the two sugars were added simultaneously the lactate production was additive. The effect of these hexoses and insulin was studied on leukocytes from normal and diabetic subjects. The responses after cell injury were also determined.

THE INFLUENCE OF GLUCOSE, FRUCTOSE, AND INSULIN ON THE METABOLISM OF LEUKOCYTES OF HEALTHY AND DIABETIC SUBJECTS 12