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In recent years the carbohydrate metabolism of the isolated rat diaphragm has been widely studied (1-8). Using this preparation, Gemmill (2, 3) succeeded in demonstrating in vitro the stimulating effect of insulin on the glucose utilization and glycogen synthesis of an isolated muscle. It has since been shown that a quantitative relationship exists between the amount of insulin present in the incubation medium and its effect on the glucose metabolism of the diaphragm (5, 6). Furthermore, it was found that the diaphragms of young rats are sensitive to extremely small concentrations of insulin (9). This investigation was intended to determine if the glucose metabolism of the isolated rat diaphragm could be used to demonstrate the presence of insulin in blood serum and to determine the concentration of this hormone in blood.

EVIDENCE FOR THE PRESENCE OF INSULIN IN BLOOD SERUM. A METHOD FOR AN APPROXIMATE DETERMINATION OF THE INSULIN CONTENT OF BLOOD 1