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The extensive administration of whole blood, plasma, and serum in the past few years has made the problem of homologous serum hepatitis an important one. The 23,000 cases of hepatitis in Armed Forces personnel (1) resulting from the injection of certain lots of yellow fever vaccine which had been stabilized with human serum gave great impetus to study of the disease in this country. Most epidemiological investigations suggest strongly that the risk of transmitting hepatitis is greater with plasma than with whole blood (2). This presumably depends on the practice of pooling the plasma from a number of donors, an occasional one of whom may harbor the virus, and of administering each pool to multiple recipients. In the preparation of pooled plasma for the Armed Services from blood collected by the American Red Cross during the war, the size of the pools was set at 25 or 50 bloods depending upon final distribution in packages of 250 ml. or of 500 ml. In the preparation of normal human serum albumin, similarly collected pools of plasma representing from 250 to 2,000 bloods were used as starting material for the process of plasma frac-

the journal of clinical investigation/the journal of clinical investigation

CHEMICAL, CLINICAL, AND IMMUNOLOGICAL STUDIES ON THE PRODUCTS OF HUMAN PLASMA FRACTIONATION. XXXVI. INACTIVATION OF THE VIRUS OF HOMOLOGOUS SERUM HEPATITIS IN SOLUTIONS OF NORMAL HUMAN SERUM ALBUMIN BY MEANS OF HEAT 12