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The distribution of sweating in normal and pathological conditions has been the subject of many investigations, and many methods for qualitative and quantitative measurement of the amount of sensible and insensible perspiration have been described. A survey of available literature has revealed fewer methods for quantitation of the number of functionally active sweat glands, and none which combine the technical simplicity and accuracy of the method herein described. Of the so-called colorimetric methods, Minor's classical starch-iodine test (1) is the most commonly used. This method is not useful in quantitative studies however, ard is subject to clinical objection, because of the necessity of application of a starch paste with a resultant black stain in a positive test. Roth (2) reported a clinical test for sweating in which active secretion is marked by a color change in cobalt chloride on the skin, and Silverman and Powell (3) report a colorimetric test involving a reaction between ferric chloride and tannic acid in the presence of active sweating. Gurney and Bunnell (4) described a method in which paper is floated over silver nitrate solution and exposed to ultraviolet light; sweating is indicated by small brown spots of reduced silver nitrate. Direct microscopic examination of active sweat pores is reported by Kuno (5), and by Lobitz and Osterberg (6), and Buley (10).

QUANTITATION AND REGIONAL DISTRIBUTION OF SWEAT GLANDS IN MAN 1