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Considerable data have accumulated on ascorbic acid levels in blood plasma (1), but reported data on the level of ascorbic acid in the white cellplatelet layer are limited (1 to 4). This is due to technical difficulties and to the quantity of blood required for a single determination. Estimations of ascorbic acid in whole blood have also been advocated as a means of reflecting the level in the white cell-platelet layer (6). Data on the relationship between the ascorbic acid level in the red cells and the plasma have also been presented (2, 7, 8). In this study, a relatively simple method is presented which permits analysis of ascorbic acid levels in whole blood, plasma, red cells, and white layer in a single 4.5 ml. specimen of venous blood, together with analyses of ascorbic acid concentrations in whole blood, plasma, red cells, and white cell layer.

the journal of clinical investigation/the journal of clinical investigation

STUDIES IN ASCORBIC ACID WITH ESPECIAL REFERENCE TO THE WHITE LAYER. I. DESCRIPTION OF METHOD AND COMPARISON OF ASCORBIC ACID LEVELS IN WHOLE BLOOD, PLASMA, RED CELLS, AND WHITE LAYER 12