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The development of the by-products, from the preparation of human serum albumin for the Armed Forces (1), made available large quantities of globulins containing isohemagglutinins. It was found possible to produce material rich in isohemagglutinins from pools of human groupspecific plasma, derived from bloods unselected for agglutinin titer. The method of preparation has been reported by Pillemer and co-workers (2). It became the problem of the Subcommittee on Blood Substitutes of the National Research Council to evaluate the product. A group of consultants,2 qualified in the field of blood grouping, collaborated in testing various lots of concentrated isohemagglutinin preparations made at the Pilot Plant, Department of Physical Chemistry, Harvard Medical School. Samples from 14 lots of material were distributed to the investigators for study. The first 6 lots were in the liquid form while the remaining 8 were converted to the dry state and sealed in vacuo. The consultants all agreed that the 14 preparations submitted for evaluation were sufficiently potent to be acceptable as grouping sera. These conclusions were based on each man's experience

CHEMICAL, CLINICAL, AND IMMUNOLOGICAL STUDIES ON THE PRODUCTS OF HUMAN PLASMA FRACTIONATION. XIV. APPRAISAL OF ISOHEMAGGLUTININ ACTIVITY 1