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Despite the numerous reports on the hormone content of human blood and urine, only a hazy concept of the actual and relative content of gonadotropic substance in urine or blood exists. As recently as June 10, 1937 the consensus at the meeting of the Section on Obstetrics, Gynecology,. and Abdominal Surgery, at the EightyEighth Annual Session of the American Medical Association seemed to be that, although the hormone assays were of scientific interest, they had little clinical value as yet. To quote two opinions concerning hormone assays, Emil Novak said, " The papers we have heard today illustrate the usual inadequacy of blood and urine hormone studies in pointing the way toward successful treatment." (1); and Elmer Sevringhaus said, "These studies are still to be reserved for the highly experimental clinics, because assay technics are far from being uniformly reliable." (2). The three most important underlying difficulties with the gonadotropic assays to date appear to be: (1), lack of adequate assay methods; (2), lack of reliable concentration methods; and (3), lack of a thorough knowledge of the normal physiology of gonadotropic hormones in the human beirng. The assay methods have recently been improved by the introduction of reliable technics using the immature mouse (3) and the immature rat (4). The problem of urine concentration has also been simplified recently by Levin and Tyndale (5) who introduced the tannic acid method, and by Heller and Heller (6) who have successfully applied Zondek's alcohol-ether concentration method. This paper attempts to add information concerning the third problem, namely, the excretion rate

GONADOTROPIC HORMONE: URINE ASSAYS OF NORMALLY CYCLING, MENOPAUSAL, CASTRATED, AND ESTRIN TREATED HUMAN FEMALES 1