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In 1923, Salvesen and Linder (1) pointed out that hypoproteinemia in the nephrotic type of Bright's disease is associated with hypocalcemia, the calcium content of the serum tending to parallel the total serum protein level. Marrack and Thacker (2) and Hastings, Murray and Sendroy (3) subsequently expressed this proportionality between total calcium and total protein more precisely in the form of an empirical regression equation. By plotting the calcium content of body fluids of low or normal protein content as ordinates against the respective total protein values, they showed that the points so obtained approximate a straight line which has a positive slope and intersects the " y " (Ca) axis at a point above the origin. Such a linear relation, since confirmed in nephrotic and normal sera by others (4, 5, 6), may be expressed by the general regression equation (5):

RELATION OF SERUM CALCIUM TO SERUM ALBUMIN AND GLOBULINS