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To describe an algorithm and software application (APP) for 100% optic nerve axon counting and to compare its performance with a semi-automated manual (SAM) method in optic nerve cross-section images (images) from normal and experimental glaucoma (EG) nonhuman primate (NHP) eyes.

Automated Quantification of Optic Nerve Axons in Primate Glaucomatous and Normal Eyes—Method and Comparison to Semi-Automated Manual Quantification