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Pseudomonas aeruginosa isolates from microbial keratitis are invasive or cytotoxic toward mammalian cells, depending on their type III secreted toxins. Cytotoxic strains express ExoU, a phospholipase that contributes to corneal virulence. This study determined whether the ExoU phospholipase domain is required for P. aeruginosa traversal of the human corneal epithelium.

Traversal of Multilayered Corneal Epithelia by CytotoxicPseudomonas aeruginosaRequires the Phospholipase Domain of ExoU