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The rumen-mimetic continuous cultivation system (RMS) was used to produce volatile fatty acids (VFAs), such as acetic, propionic, and butyric acids, from lignocellulosic biomass. Typical 12 species of ruminal bacteria were detected in the culture broth cultivated by RMS using species-specific PCR assay. These species are known to be cellulolytic, hemicellulolytic, proteolytic, amylolytic, and lipolytic bacteria and methanogens. Therefore, RMS was characterized to be ruminal fermentation like the digestive system of roughage in the rumen of cattle. Using RMS, the ruminal bacteria were continuously cultivated for 120 days to produce VFAs. The yield of total VFAs from 20–50 μm rice straw was 167% higher than that from 300–1000 μm rice straw. However, the productivity was not increased by pulverization of the substrate. The yield and productivity of acetic acid from 20–50 μm rice straw were 0.147 g/g and 34.8 mg/L/h, respectively, and those of propionic acid were 0.104 g/g and 24.6 mg/L/h, respectively. The selectivity (P/A ratio) of acetic and propionic acid production from microcrystalline cellulose at pH 6.5 and 7.0 were 0.83±0.07 and 0.31±0.03, respectively. The ruminal bacteria could not continue to produce VFAs at pH 6.0. The yield and productivity of acetic acid from microcrystalline cellulose at pH 6.5 were 0.234 g/g and 51.7 mg/L/h, respectively, and those of propionic acid were 0.223 g/g and 49.3 mg/L/h, respectively. This study proposes that in order to increase the productivity of RMS, it is necessary to increase the ruminal bacterial biomass by recycling biomass (flocs) using a settling tank.

Characterization of the Rumen-Mimetic Continuous Cultivation System for Volatile Fatty Acid Production from Lignocellulosic Biomass