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P73 Background: Elevated macromolecule signals, as  measured by proton magnetic resonance spectroscopy (MRS) after human  stroke, are hypothesized to arise from mobile lipids that accumulate  within macrophages. However, their chemical identity, cellular  location, and clinical significance remain uncertain.Methods: Adult Wistar rats underwent a 90 minute middle  cerebral artery occlusion with reperfusion. Animals survived for 2 to 5  days prior to sacrifice. Immediately after decapitation, 400 μm  slices from an infarcted region of forebrain were prepared and perfused  in artificial CSF at 36 degrees C, and studied by gradient-enhanced  one-dimensional (1D) MRS and by two-dimensional correlation  spectroscopy (COSY). Cryostat coronal sections from other, identically  prepared rats were stained for lipid (Nile Red) and immunostained for  microglia/macrophages (OX42).Results: The 1D spectra from  infarcted samples contained broad resonances arising from lipid  methylene and other fatty acyl protons that were absent in control  samples. These assignments were confirmed by the COSY crosspeaks.  Increases in crosspeaks from mobile polypeptides were not detected. The  corresponding histologic specimens showed abundant lipid droplets  within the infarct that were not seen in contralateral, uninfarcted  tissue. These droplets were primarily located within OX42  immunostaining cells.Conclusions: Our model studies support  the hypothesis that macromolecule signals detected by MRS in subacute  stroke arise from mobile lipids concentrated in macrophages. These  results help to clarify the spectroscopic findings in humans. Further  histologic study is warranted to determine whether cells containing  lipid droplets that are not stained by OX42 are also  present.

Spectroscopy and Histology Demonstrate Lipids in Ischemic Rat Brain after Middle Cerebral Artery Occlusion with Reperfusion