Open AccessJmjd3 Controls Mesodermal and Cardiovascular Differentiation of Embryonic Stem Cells
Author(s) -
Kisho Ohtani,
Cong Zhao,
Gergana Dobreva,
Yosif Manavski,
Britta Kluge,
Thomas Braun,
Michael A. Rieger,
Andreas M. Zeiher,
Stefanie Dimmeler
Publication year - 2013
Publication title -
circulation research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.899
H-Index - 336
eISSN - 1524-4571
pISSN - 0009-7330
DOI - 10.1161/circresaha.113.302035
Subject(s) - brachyury , mesoderm , fgf and mesoderm formation , biology , embryonic stem cell , wnt signaling pathway , microbiology and biotechnology , nodal , cellular differentiation , stem cell , lateral plate mesoderm , genetics , cancer research , signal transduction , gene
Rationale: The developmental role of the H3K27 demethylases Jmjd3, especially its epigenetic regulation at target genes in response to upstream developmental signaling, is unclear. Objective: To determine the role of Jmjd3 during mesoderm and cardiovascular lineage commitment. Methods and Results: Ablation of Jmjd3 in mouse embryonic stem cells does not affect the maintenance of pluripotency and self-renewal but compromised mesoderm and subsequent endothelial and cardiac differentiation. Jmjd3 reduces H3K27me3 marks at theBrachyury promoter and facilitates the recruitment of β-catenin, which is critical for Wnt signal–induced mesoderm differentiation.Conclusions: These data demonstrate that Jmjd3 is required for mesoderm differentiation and cardiovascular lineage commitment.
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