Open AccessA Procedure for the Quantitative Determination of Protein of the Rat Heart
Author(s) -
H. Sobel,
Sara M. Myers,
Jessie Marmorston
Publication year - 1955
Publication title -
circulation research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.899
H-Index - 336
eISSN - 1524-4571
pISSN - 0009-7330
DOI - 10.1161/01.res.3.5.454
Subject(s) - ionic strength , extraction (chemistry) , chromatography , chemistry , centrifugation , differential centrifugation , phosphate , phosphate buffered saline , electrophoresis , sucrose , pyrophosphate , biochemistry , enzyme , aqueous solution , organic chemistry
A procedure has been developed for the quantitative determination of the extractable proteins of the rat heart. Utilizing extraction with a sucrose-versene solution and employing differential centrifugation, followed by extraction with a phosphate-pyrophosphate buffer, low-ionic-strength extractives, a mitochondrial fraction, and high-ionic-strength extractives have been obtained. Electrophoretic analysis has shown five subfractions to be present in the low-ionic-strength extractives and three sub-fractions in the high-ionic-strength extractives.
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