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Isolated rabbit hearts were perfused with a suspension of red cells which assured adequate oxygenation. Ouabain added to the perfusate (2.8 x 10-7 M final concentration) promptly increased the force of contraction and oxygen consumption of the hearts. These events appeared to occur simultaneously. The extraction ratio of palmitate-1-14 C added to the perfusate (0.31) was not altered by ouabain. However, ouabain increased the oxidation of the extracted palmitate from an average of 34% to an average of 71% at the time intervals studied.

Effects of Ouabain on Force of Contraction, Oxygen Consumption, and Metabolism of Free Fatty Acids in the Perfused Rabbit Heart