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We have previously reported that leukemia inhibitory factor (LIF) gradually increased cardiac L-type Ca2+ channel current (I CaL ), which peaked at 15 minutes in both adult and neonatal rat cardiomyocytes, and this increase was blocked by the mitogen-activated protein kinase kinase inhibitor PD98059. This study investigated the molecular basis of LIF-induced augmentation ofI CaL in rodent cardiomyocytes. LIF induced phosphorylation of a serine residue in the α1c subunit (Cav 1.2) of L-type Ca2+ channels in cultured rat cardiomyocytes, and this phosphorylation was inhibited by PD98059. When constructs encoding either a wild-type or a carboxyl-terminal–truncated rabbit Cav 1.2 subunit were transfected into HEK293 cells, LIF induced phosphorylation of the resultant wild-type protein but not the mutant protein. Cotransfection of constitutively active mitogen-activated protein kinase kinase also resulted in phosphorylation of the Cav 1.2 subunit in the absence of LIF stimulation. In in-gel kinase assays, extracellular signal–regulated kinase phosphorylated a glutathioneS -transferase fusion protein of the carboxyl-terminal region of Cav 1.2 (residues 1700 through 1923), which contains the consensus sequence Pro-Leu-Ser-Pro. A point mutation within this consensus sequence, which results in a substitution of alanine for serine at residue 1829 (S1829A), was sufficient to abolish the LIF-induced phosphorylation. LIF increasedI CaL in HEK cells transfected with wild-type Cav 1.2 but not with the mutated version. These results provide direct evidence that LIF phosphorylates the serine residue at position 1829 of the Cav 1.2 subunit via the actions of extracellular signal–regulated kinase and that this phosphorylation increasesI CaL in cardiomyocytes.

circulation research

Leukemia Inhibitory Factor Activates Cardiac L-Type Ca <sup>2+</sup> Channels via Phosphorylation of Serine 1829 in the Rabbit Ca <sub>v</sub> 1.2 Subunit

Leukemia Inhibitory Factor Activates Cardiac L-Type Ca 2+ Channels via Phosphorylation of Serine 1829 in the Rabbit Ca v 1.2 Subunit

Leukemia Inhibitory Factor Activates Cardiac L-Type Ca ²⁺ Channels via Phosphorylation of Serine 1829 in the Rabbit Ca _v 1.2 Subunit