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A simple method, with use of intravenous fluorescein, was used for producing and photographing fluorescence in circulating blood of the human retina. Separate arteriolar and venous filling phases, arteriolovenous shunt, sluggish choroidal circulation, stratified flow of fluorescein, and rapid central retinal circulation times were observed in normal retinas. Similar findings were seen in hypertensive and diabetic patients, and, in addition, neovascularization was clearly defined, and some cotton-wool patches, but not hemorrhages, were found to fluoresce. Limitations and applications of the method are discussed.

A Method of Photographing Fluorescence in Circulating Blood in the Human Retina