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To the Editor,In a recent article, Taylor et al1 have shown that C-Reactive Protein (CRP), per se, does not activate endothelial cells; rather, lipopolysacchride (LPS) and azide were responsible for the observed cellular activation. We would like to provide our data to further support the conclusion by Taylor and colleagues.We have tested >10 batches of commercial human CRP preparations from 3 companies (Calbiochem, TriChem, and Sigma). Human saphenous vein endothelial cells (HUVECs, passages 3 to 5) were grown …

Lipopolysaccharide (LPS) Contamination Plays the Real Role in C-Reactive Protein–Induced IL-6 Secretion From Human Endothelial Cells In Vitro