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Endothelium-derived hyperpolarizing factor (EDHF) plays an important role in modulating vascular tone, especially in microvessels, although its nature has yet to be elucidated. This study was designed to examine whether hydrogen peroxide (H2O2) is an EDHF in porcine coronary microvessels with use of an electron spin resonance (ESR) method to directly detect H2O2 production from the endothelium.

Electron Spin Resonance Detection of Hydrogen Peroxide as an Endothelium-Derived Hyperpolarizing Factor in Porcine Coronary Microvessels