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Bacteriocin 2a produced by Lactobacillus sake 2a isolated from a Brazilian meat product (lingüiça) was purified, characterized and its mecanism of action was studied. The bacteriocin 2a showed bactericidal effect against Listeria monocytogens, Listeria we/shimeri, Listeria see/igeri and Listeria inocua but it did not have an effect against Staphy/ococcus aureus e Escherichia colí 0157H7 strains. The highest concentration of bacteriocin 2a (450 AU/ml) was found in MRS medium incubated at 25 or 30°C and its production was at its maximum towards logaritmic growth. It was stable at 121° C for 15 minutes. Bacteriocin 2a was purified 71186 fold by salt extraction from Lactobacillus sake cells, followed by cation exchange chromatography using Mono S column. It has an estimated molecular mass of 3-6kDa. In mechanistic studies of action against Listeria monocytogenes Scott A, bacteriocin 2a dissipated the pH gradient, the .L\\f' and reduced the ATP internai concentration by 80% with no detectable increase in the externai ATP concentration. Bacteriocin 2a concentration of 28, 60 and 114 AU/ml, also mediated carboxyfluorescein efflux of 3, 30 and 100% from liposomes made from lipids extracted from Listeria monocytogenes Scott A, respectively. These data indicate that bacteriocin 2a forms pores in the citoplasmatic membrane of target cells similarly Class 11 bacteriocins. In addiction it can be use as a potential anti-microbial against Listeria monocytogenes in food .

Purificação e mecanismo de ação de uma bacteriocina produzida por Lactobacillus sake 2a isolado de linguiça frescal