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In the region of Suzano, a wide range of industrial and domestic wastes are discharged in the River Tiete. This region deserves special attention because of its proximity to the origin of the river. Biological treatment of effluents is carried out by WWTP (Wastewater Treatment Plant of Suzano) of SABESP (Companhia de Saneamento Basico do Estado de Sao Paulo). The aim of the study was evaluate the potential impact of secondary effluent discharge on Tiete River by analyzing acute toxicity and mutagenicity in the freshwater snail Biomphalaria glabrata. Acute toxicity assays were conducted in adult specimens and embryo at blastulae, gastrulae, trocophore and veliger stages. Dominant lethal test was performed for detection of germ cell mutations. Results obtainded in acute toxicity test were used to determine the range of concentration for the dominant lethal test. After the end of the exposure, adults wild-type snails were crossed with non-exposed albino snails at different intervals to evaluate the effects in the germ cells at the different stages of spermatogenesis  10, 17, 24, 31, 38, 45, 52 and 59 days. The frequency of malformations on the wild embryos of the albino offspring was used as indicator of germ cell mutations. Samples were collected and transported by SABESP. The following points were chosen: P0  Ponte Nova Dam, P1  200 meters upstream to the plant, P2  the affluent of plant, P3  the effluent treated by plant before discharge in the river and P4  200 meters downstream to the discharge in the river. Four sampling were performed: two samples in winter (C1  August of 2006 and C3  August of 2007) and two in summer (C2  February of 2007 and C4  March of 2008). At August 2006 and August 2007, the station affluent was toxic for embryos and adults. The results were similar, with LC50 values; 43.04%, 41.56%, 57.16% and 60.06% for embryos at blastulae, gastrulae, trocophore and veliger stages respectively and 100% for adult snails in first sample and 48.24%, 43.71%, 55.43% and 62.64% for embryos at blastulae, gastrulae, trocophore and veliger stages respectively and 84.16% for adult snails in august

Avaliação dos efeitos tóxicos e mutagênicos de amostras ambientais do Rio Tiête na região de Suzano em >i/i< (SAY, 1818)