English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Tools annual

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Presents the access of premium content as premium feature

Premium Content

Global: Zendy Plus annual

Global: Zendy Free Plan

The use and development of biomaterials for tissue regeneration are of great importance, especially for medical and dental care. Collagen ma trices derived from animal tissues are widely used because collagen has characteristics su ch as biodegradability and biocompatibility. These matrices can be obtained fr om various sources, such as porcine pericardium, which is a tissue that can be used due its low cost, wide availability and because it can be chemically modified. Besides, porcine tis sues are very similar to human tissue and can be used to produce biomaterials for soft tissue reg neration. The aim of this study was the preparation and characterization acellular membrane s by alkaline hydrolysis of porcine pericardium. Membranes were characterized by histol ogical analysis, scanning electron microscopy (SEM), in vitro cytotoxicity evaluation, in vitro biological stability (collagenase), potentiometric titration, water absorption percenta ge, differential scanning calorimetry (DSC), thermogravimetry (TG), dynamic mechanical thermal a nalysis (DMTA) and tensile tests. Histological analysis showed that after 4h of hydro lysis, cells were totally removed from matrices. In vitro cytotoxicity showed that all matrices prepared in t his work are not cytotoxic. In vitro biological stability tests (collagenase) showed th at the hydrolyzed membranes degraded more quickly than the non hydrol ized matrix and more resistant to collagenase degradation when compared to matrices d erive from bovine pericardium. The potentiometric titration allowed the determination carboxylic groups and the increase of these groups per collagen molecule. Hydrolyzed matrices h ad an increase in water absorption, a decrease in denaturation temperature and a small de crease in thermal stability with the increase of hydrolysis time. Tensile tests showed t hat after alkaline hydrolysis matrices showed higher tensile strength and the deformation was independent of the time of alkaline hydrolysis. These results showed that the preparati on of collagen biological matrices derived from porcine pericardium with different times of al k line hydrolysis is a viable procedure to be subsequently used in the production of biomateri als for tissue engineering.

Desenvolvimento de membranas acelulares de colágeno derivadas de pericárdio porcino para uso em engenharia de tecido