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GALUPPO, M. K. Functional characterization of CD100 / SEMA4D in macrophage infection by Leishmania (Leishmania) amazonensis. 2015. 99 p. Ph. D. thesis (Parasithology) Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, 2015. Leishmaniasis is caused by trypanosomatid protozoa of the genus Leishmania that infect preferentially macrophages. The disease may present in cutaneous, mucocutaneous and visceral forms, and 12 million people worldwide are infected. Several factors influence the form and severity of the disease, and the species of Leishmania and the host immune response are the main ones. Studies based on models of infected animals susceptible and resistant to Leishmania show how the host immune system with different profiles affects the survival of the parasite. Considering the importance of the macrophage profile in Leishmania infection, the potential role of CD100 (shown to be expressed by macrophages from BALB/c and C57BL/6) in the modulation of macrophage activation and our data that recombinant soluble CD100 (sCD100) enhances the infectivity of that cell by the parasite, our objective was the functional characterization of CD100 on the infection of macrophages by Leishmania (Leishmania) amazonensis. For this, we analyzed the expression, location and role of the protein in macrophages infected or not by L. (L.) amazonensis, as well as in peritoneal macrophages from C57BL/6 knockout for this molecule. We observed that sCD100 increases the infection and phagocytosis of promastigotes of L. (L.) amazonensis and Zymosan particles by murine peritoneal macrophages. We demonstrated that this modulation occurs when sCD100 interacts with the macrophage through its CD72 receptor. We showed that the infectivity of L. (L.) amazonensis promastigotes in vitro is similar in wildtype and CD100-knockout macrophages, but that knockout animals develop smaller lesions in vivo when compared to wildtype animals. Keyeword: Leishmania (Leishmania) amazonensis. Sema/4D. CD100. Macrophage.

Caracterização funcional de CD100/Sema4D na infecção de macrófagos por Leishmania (Leishmania) amazonensis.