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KORTE, R. L. Survey of Bancroftian filariasis infection in humans and Culex mosquitoes in the western Brazilian Amazon region: implications for transmission and control. 2013. 105 p. Ph. D. Thesis (Biology of Host-Pathogen Interactions) – Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, 2013. Introduction: This work is aimed to identify possible lymphatic filariasis foci in the Western Brazilian Amazonian rainforest that could be established since the reports of Rachou and cols in 1950’s, and as well find possible presence of Culex quinquefasciatus with natural infection by Wuchereria bancrofti. Porto Velho and Guajara-Mirim (Rondonia State) and Humaita (Amazonas State), all in the north region of Brazil, were the study target area. Methods: Blood thick film method with samples collected from 10 PM to 1 AM were stained using Giemsa to evaluate human infection and Polymerase Chain Reaction for W. bancrofti DNA in mosquitoes vectors. Samples from the neighborhoods of people over 5 years old of areas lacking sanitation systems and also samples from students attending public nighttime classes in the cities referred to above were analyzed and evaluated, as well as mosquitoes captured in the houses. Results: Nine hundred and thirty-five individuals (54.4%) out of a total of 1,720 individuals engaged were evaluated based on the domicile probes. Sample with night students involving 2,709 individuals (75.2%) out of the total of 3,601 previously selected. No individuals were positive for the presence of microfilariae in the blood stream. Seven thousand eight hundred and forty nine female Culex quinquefasciatus specimens were captured and after evaluated by using PCR method, all of them were found negative for Wuchereria bancrofti DNA. Conclusion: By carrying out haemoscopic examination of the human population samples and the mosquito vectors, the conclusion is that actually such target areas are not affected by W. bancrofti transmission.

Filariose bancroftiana na Amazônia Ocidental brasileira: implicações para transmissão e controle.