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Siriani LK. Influence of cnm gene of Streptococcus mutans in biofilm formation and interaction to endothelial cells. [Ph. D. thesis (Microbiology)]. São Paulo: Instituto de Ciências Biomédicas, Universidade de São Paulo; 2012. Streptococcus mutans is considered a primary etiological agent of dental caries and an important etiological agent of infectious endocarditis. It can be classified into four serotypes (c, e, f and k), which serotype c is the most prevalent in the population. S. mutans may also have access to the bloodstream, which they may infect the endothelial cells of the coronary arteries by binding to and subsequently invading them. The recent breakthrough of cnm gene, which encodes a collagen binding protein found in some S. mutans strains, has set off new studies on this species, such as biofilm formation and bacteria-cell interactions. This study aims investigating the role of the cnm gene in the abilities of S. mutans: (1) biofilm formation on collagen-treated surfaces and the expression of biofilm formation key genes spaP and brpA; (2) adhesion (30 min and 2 h), invasion (5 h) and persistence (24 h) in endothelial cells and (3) dentin demineralization. Experiments on collagen-dependent biofilm formation, and adhesion, invasion and persistence in primary Human Coronary Artery Endothelial Cells (HCAEC) were performed using S. mutans strains UA159 (serotype c), B14 (serotype e), OM50E (serotype e), LM7 (serotype e), OMZ175 (serotype f), NCTC 11060 (serotype f) and the clinical isolates of this study 7.1 (serotype c), C2A4 (serotype e) and 61 (serotype k). Mutants cnm were constructed in all strains presenting the gene, except for C2A4. Strains OMZ175 and 61 and their respective mutants were also employed to evaluate both the expression of spaP and brpA genes by PCR Real Time and the dentin demineralization through Optical Coherence Tomography (OCT). One hundred forty four clinical isolates of S. mutans were collected from 47 patients. Of the clinical isolates, 138 out of 144 were identified as serotype c, 1/144 was identified

Influência do gene cnm de Streptococcus mutans na formação de biofilme e na interação do microrganismo às células endoteliais.