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Yshii LM. Activation of trasnscription fator kB induced by alpha-synuclein in SHSY5Y cells [Ph.D. thesis (Pharmacology)]. São Paulo: Instituto de Ciências Biomédicas da Universidade de São Paulo; 2011. Parkinson’s Disease (PD) is a neurodegenerative disease. The characteristics and symptoms are well defined; nevertheless its etiology remains unknown. The sporadic PD is characterized by the presence of Lewy Body (aggregate of proteins) inside the neurons. Alpha-synuclein is a soluble protein present in the pre synaptic terminal of neurons. Evidences suggest that this protein is a fundamental component of Lewy bodies localized in the dopaminergic neurons of PD patients. It is already known that alpha-synuclein has a fundamental role in pathogenesis of PD, because it can affect the homeostasis of dopaminergic neurons, leading to increase of dopamine in the cytosol and consequent oxidative stress. The nuclear transcription factor kB (NF-kB) regulates the immune, inflammatory and cell death responses. In the central nervous system, this factor is present in several types of cells and its role is paradoxal, since it can be neurotoxic or can be protective. The NFkB can be stimulated by several factors, including dopamine, glutamate, stress and β-amyloid protein. In this work, we observed the molecular modification in SHSY5Y cells transduced with alpha-synuclein (wild-type, A30P and truncated 1-120) and treated with conditioned medium (CM) (from primary culture of glia treated with LPS) or TNF. We analyzed the modulation of NF-kB activity, in which was observed that the activity was increased when the cells were treated with TNF but not with CM. Moreover, we show that there is a decrease of MAPK42/44 phosphorilation during the treatment, and this decrease is linked to the increase of cell death of these cells overexpressing alpha-synulein.

Efeitos da alfa-sinucleína na modulação da atividade do fator de transcrição nuclear kB em células SH-SY5Y.