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xx Resumoxxi Cap.1 Introdução 1 1 Substâncias contaminantes no ambiente2 2 O que é Ecotoxicologia5 3 Relevância ecológica 6 4 Análise Integrada (AI) e Avaliação de Risco Ecológico (ERA) 7 5 Tríade de Qualidade de Sedimentos e “Weight-of-Evidence” (WOE)8 6 Escolha do Local de Estudo10 7 Objetivo 10 8 Referências11 Cap.2 General Introduction to Methodology14 1 Sediment Quality Guidelines (SQG) and Integrated Assessment14 2 Sampling Area17 3 Methodology20 3.1 Digestion and Measurement of AVS and SEM 20 3.1.1 Organic Carbon Calculation 21 3.2 Water Column 22 3.3 Extraction 22 3.4 Battery of Bioassays24 3.4.1 RTL-W1 cell and cell culture 24 3.4.2 Cytotoxicity Assay – Neutral Red 26 3.4.3 Alkaline Comet assay 27 3.4.4 Micronucleus assay 29 3.4.5 Ethoxyresorufin-O-deethylase (EROD) activity 32 3.4.6Ames Fluctuation Test33

Variação temporal do teor de SVA/ MES e avaliação integrada do sedimento do braço do Rio Grande (Complexo Billings - SP)