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Phenolic compounds related to resistance of the coffee tree at coffee leaf miner (Leucoptera coffeella ) and at the rust (Hemileia vastatrix ) The plants present different and complex mechanisms of defense, that work together, in responses to biotic and abiotic stresses, whose nature and intensity of response varies with age, the degree of adaptation and phenology. The main objective from the research is: (i) detect the chlorogenic acids in the leaves of Coffea arabica L., cultivars Obatã IAC 1669-20 and Catuaí Vermelho IAC 99, and of the populations into selection H14945-46 and H20049, and (ii) quantify the several classes of chlorogenic acids, in the same coffee plants, during the fructification phases of the coffee tree (flowering, fruits at the beginning of growth , grain expansion/seed, grain maturation). The phenolic compounds have been apart through high performance liquid chromatography (Shimadzu , model LC -20A) for the analyses in CLAE DAD. The studied chromatography profile of the coffee plants has presented no difference between them. During the fructification phases there was variation at the content of chlorogenic acid and of the total phenols, in which has presented under age values at the phase as of grain expansion. The plants of H14954-46 resistant to the coffee leaf miner have presented the chlorogenic acid referring to pico 5, no common to the other coffee plants studied. The infested leaves by coffee leaf miner (Leucoptera coffeella) presented lower concentrations of total phenols, as well as, of some chlorogenic acids and flavanoids, unlike, the leaves inoculate by rust (Hemileia vastatrix) presented greater concentration. There is evidence that chlorogenic acids have involved in the complex defense mechanism of plants.

Compostos fenólicos relacionados à resistência do cafeeiro ao bicho-mineiro (Leucoptera coffeella) e à ferrugem (Hemileia vastatrix)