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Of the original 1020 participants, 601(381 men/220 women) had DNA samples collected. Genomic DNA was isolated from peripheric blood leukocytes using salting-out methodology. The TaqMan allelic discrimination method was employed for genotyping, using specific probes. The analyzed SNPs were 1818 C>T (rs564481), 395 G>A (rs1207568), and 1117 G>C (rs9527025), all chosen due to previous data in literature.

revista de medicina

KLOTHO 1818T polymorphism associated with myocardial infarction, abdominal aortic calcification and lower creatinine clearance in community-dwelling older subjects: the Sao Paulo Ageing &amp; Health Study (SPAH)