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SANTOS, F. A. O. Evaluation of difference immune response in neonatal mice using outer membrane vesicles of Neisseria meningitidis B complexed with two different adjuvants. 2017. 92 f. Masters thesis (Biotechnology) – Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, 2017. Adjuvants are molecules, compounds or macromolecular complexes that increase the power and longevity of the specific immune response to antigens. Their addition in the vaccine preparation increase sustains and directs immunogenicity of antigens, effectively modulating appropriate immune responses, reducing the amount of antigen or number of immunizations required, and improving the efficacy of vaccines in infants, the elderly, or immunocompromised patients. The aim of this study was to evaluate the immunogenicity of antigenic from outer membrane vesicles (OMVs) of N. meningitidis B complexed with two different adjuvants: DODAB-BF and aluminium hydroxide (Al(OH)3) comparing the evaluation of subcutaneous and intranasal route of immunization for the first time using the prime-boost system in outbred neonatal mice. As universal methods of antibody detection were used: Immunoblot, DOT-ELISA, ELISA and ELISpot aiming for the humoral and cellular immune response and of male and female mice. By Immunoblot analysis the specificity of antibodies with the homologous strain N. meningitidis B:4P:1.19,15. By DOTELISA was verified the cross-reactivity with DODAB-BF to different serogroups (B, C, W and Y) that was not observed with alum. By ELISA the antibodies titers were quantified and compared in the sera of mice immunized with DODAB-BF+OMVs and alum+OMVs for IgG, IgG1 and IgG2a. The immunization routes used exhibited IgG titers, and both adjuvants promoted the production of IgG1 and IgG2a varying according to the route of immunization used. By ELISpot was analyze IFN-γand IL-4 and the results showed the response directly to Th1 and Th2 profile.

Avaliação da diferença da resposta imune em camundongos neonatos utilizando antígenos de membrana externa de &lt;i&gt;Neisseria meningitidis &lt;/i&gt;B complexados com dois diferentes adjuvantes.