English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

MORAIS, B. P. Study of in vitro differentiation of human immature dental pulp stem cells into retinal neurons cells. 2012. 96 p. Masters thesis (Biotechnology) Instit uto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, 2012. Introduction: Retina is the light-sensitive eye tissue which conv erts captured energy in image by specialized neuronal cells. If these cells are damaged they are unable to regenerate. Treatments aim only to decrease retinal damages ins tead of promoting an effective vision recovery. Stem cells (SC) are a promissive source t o substitute injured tissue. Immature dental pulp stem cells (IDPSC) have characteristics of plu ri otent SC and are able to acquire properties of almost all cell types. Objectives: We aim to evaluate the potential of IDPSC to develop retinal neurons, which can represent a new source for treatment of retinal degenerative diseases. Methods: Undifferentiated IDPSC, previously established by u s, were analyzed by immunocytofluorescence to evaluate CD73 expression, an early photoreceptor marker. Further, these cells were submitted to prog enitor neural differentiation. The capacity of IDPSC to differentiate towards retinal spheres s imilar structures was evaluated by the expression of Nestin, β-III-tubulin and Pax-6 antibodies. Retinal cells di fferentiation was perfomed from retinal spheres and was evaluated by the expression of specialized retinal neurons antibodies: Pax-6, Chx-10, Crx, Nrl, Calbin din, Recoverin and Rhodopsin. Results: Undifferentiated IDPSC reacted positively to CD73. We also observed the positive reaction of anti-nestin and antiβ III-tubulin in retinal spheres, indicating that th ese structures have previous commitment with neural lineage. Anti-Pax-6 , a marker of progenitor retina cells, was also positive in retinal spheres. Regarding other s p cialized retinal markers, they did not show any positive reaction in formed spheres. On the oth r hand, in differentiated cells from retinal spheres, we observed a high expression of Chx-10 an d Crx, in comparison to Nrl, Calbindin, Recoverin and Rhodopsin, which were also positive b ut less intensive. We confirmed that CD73 was also expressed in these differentiated cel ls obtained from retinal spheres. Conclusion: This study suggests that IDPSC present the potentia l of developing retinal spheres structures, with neural and retinal propert ies in vitro, which can be induced to differentiate into retinal neurons. Our data demons trated that IDPSC can be an alternative source to regenerate damaged retinal tissues. Never th less, it is still required some factors that can facilitate the induction of mature retinal char acteristics. Further studies are needed in order to elucidate respective roles of retinal tiss ue formation.

Estudo in vitro do potencial de diferenciação das células tronco imaturas da polpa dentária humana em neurônios retinais.