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Several studies have shown that some snake venom components have efficient antitumor activity in vitro and in vivo. The venom of the rattlesnake Crotalus vegrandis has neurotoxic, myotoxic and hemorrhagic activities. The crotoxina-like is an important component of this venom and is formed by an acidic protein (crotapotin) combined to a basic subunit (PLA2). As there are few studies on Crotalus vegrandis venom, the isolation and characterization of its components and an investigation of their potential and efficacy as a therapeutic agent against tumor cells would be of great value. In this study, we carried out the isolation and characterization of the crotoxin-like of Crotalus vegrandis and assayed its cytotoxicicity on L929 and B16F10 cells. We carried out the cultivation of these cells in 96 well plates, to then be placed in direct contact with the different purified fractions Crotalus vegrandis venom for a total time of 48 hours. For comparison, we carried out the same test with the purified fractions of Crotalus durissus terrificus venom. The MTT assay was used to assess the cell viability after treatment with fractions of Crotalus vegrandis and Crotalus durissus terrificus venoms. The results showed a high cytotoxic activity for both venoms in B16F10 tumor cells and little toxicity to normal L929 cells. Also in comparative tests with both venoms, we observed that for a given concentration, the fractions of the venom of Crotalus vegrandis had higher cytotoxic effect than the Crotalus durissus terrificus venom, with greater specificity for B16F10 cells.

Isolamento e caracterização da crotoxina símile do veneno de <i>Crotalus vegrandis</i> com atividade antitumoral