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The sediment of Sao Paulo state has been evaluated by using, among other lines of evidence, results obtained from ecotoxicological tests using the amphipod Hyalella azteca. International protocols such as the USEPA, ASTM, OECD and Environment Canada describe procedures for culturing and tests with other benthic organisms such as insect larvae of the genus Chironomus sp (Insecta, Diptera). In Brazil, several studies were conducted with the species Chironomus sancticaroli without the use of a national standardized methodology. The protocols describe methods for testing the sensitivity to the reference toxicant as quality assurance and do not describe other methodologies for monitoring the biological variables in culturing of test organisms, or establish acceptability criteria. In order to ensure the quality of the organisms cultured in the laboratory and adapt a methodology for sediment, tests were conducted to establish the variation of biological variables in culture and determine an acceptability criteria for these variables. Tests with sediment samples from rivers and reservoirs from Sao Paulo state were performed during the years 2006 to 2009. Variables such as survival, menthum deformity, fecundity, fertility and hatching rate were monitored and the acceptability criteria for each one was established using percentile calculations. Variations in the monitoring of variables results suggest the existence of possible interferences such as water quality and inbreeding in culturing. The acceptability criteria determined, based on the percentile, will serve as a tool for quality control of culturing organisms under specific conditions to each laboratory. The adapted test methodology showed good performance, exceeding the acceptability criteria established to organism survival in the control test. The high sensitivity of chironomids when compared to amphipods emphasizing the existence of different responses to physicochemical and chemical characteristics of the sediment, associated with the biology of each organism, indicating that the combined use of these organisms in sediment quality assessment can be recommended.

Chironomus sancticaroli - do cultivo em laboratório ao ensaio ecotoxicológico com amostras ambientais de sedimento