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Specific RP-HPLC conditions for the analysis of recombinant and native hLH and hCG preparations and of their subunits were set up. Heterodimeric hLH and hCG and their αand βsubunits all migrated with significantly different retention times (tR) in the following order of increasing hydrophobicity: α-hCG < α-hLH < hCG < hLH < βhCG < β-hLH. With basis on these conditions, a total of eleven preparations were studied: the International Standard of recombinant hLH-WHO 96/602, a commercial recombinant and two highly purified pituitary hLH, a recombinant and two urinary hCG preparations and four heterogeneous urinary products containing hLH + hFSH. All hLH preparations showed very similar retention times for the main peak (t R R = 38.35 ± 0.42 min; RSD = 1.1 %; n = 4 preparations), while the hCG main peak ran about 4 % faster when compared to this average value. Human LH, hFSH and hCG peaks could also be identified in the heterogeneous urinary preparations. Quantitative analysis could be validated for the seven homogeneous preparations and accuracy, precision and sensitivity were calculated on the basis of a highly linear dose-response curve (r=0.99998; p<0.0001; n=20). Quantification of the differents gonadotropins in the heterogeneous urinary preparations was also carried out, though with clear accuracy limitations.

Análise de luteotrofina humana e de gonadotrofina coriônica humana, recombinante e natural, por cromatografia líquida de alta eficiência em fase reversa