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DEVELOPMENT OF LYOPHILIZED KIT OF TIN-GLUCOHEPTONATE FOR IN VITRO LABELING OF LEUCOCYTES WITH ^^^c Rosemeire Fagundes Nascimento The study and localization of inflammatory and infection process in Nuclear Medicine represents a relevant tool in diagnostic procedures. In same cases, the diagnostic is easy and based on anamneses and clinical observation; in other cases, the patients are assimptomatic or present non specific symptoms that difficult the diagnostic. The early diagnostic of inflammatory or infectious process allow the early introduction of therapy and prevents complications. Farther, the differentiation between inflammation and infection is of extreme importance as well as the localization of the focus. The use of labeled leucocytes, studied and applied in much pathologies, is the method of choice for the visualization of inflammation and infection. The scintigraphy using labeled leucocytes was introduced at 1976 by McAffe and Thakur and since of this is used in the diagnostic of different pathologies related to leucocyte infiltration like intestinal inflammatory disease, bone or prosthetic-vascular infections. The in vitro labeling of leucocytes with ^^^In was performed using oxime or tropolone as ligand and with ^^"^Tc using hexametylpropylene amine oxime (HMPAO) as ligand, resulting in a lipophilic complex. The ^^""Tc-HMPAG complex was preferably employed in many indications and countries do to the ideal physical properties of ^ ""Tc that results in low dose to the patient. However, the labeling employing the HMPAO complex results in some disadvantages like the low stability of the complex, and some requirements related to the ^ ' Tc elution (like the time pos elution), beyond the high cost of the compound that is imported. The aim of this work was the development of a tin-glucoheptonate lyophilized kit for in COMISSAO KAClOt Al 13E R^r f^ f M^JClLAB'SP-iPBi vitro leucocytes labeling with ^^'"Tc using the pre stannization method. The optimization of the labeling technique was developed using leucocytes isolated from total blood and employing different volumes of the tinglucoheptonate reagent and different incubation times at 37°C (prestannization), and posterior labeling with ^^'^Tc (185 MBq), after 20 minutes reaction at room temperature. The labeling yield was superior to 90% using the optimized labeling conditions. Lyophilized reagent was stable after 90 days. Scintilographic images obtained 1, 2 e 3 hours after the administration of labeled leucocytes in New Zeland rabit, showed good uptake on inflammatory focus promoted by tupertine injection. The leucocytes labeling method developed can be probably applied in clinical procedures and represents cost effective method in substitution of the lypophilic complex of HMPAO. C0MIS5A0 NACiOHAL DE E N E F ^ MUC!IAR/SP-'PE^

Desenvolvimento de reagente liofilizado de glucoheptonato-estanho para marcação de leucócitos com Tecnécio-99m in vitro