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The main objective of this work was the use of the agroindustrial residue from the extraction of the annatto pigment for the elaboration of biodegradable films. Three methods (water milling (WS), acid pH (AS) grinding and alkaline pH (KS) milling were used for insulation of urucum flour. The residue and flours were characterized in centesimal composition, color, particle size distribution, X-ray diffraction, scanning electron microscopy, infrared spectroscopy, differential scanning calorimetry, phenolic compounds content, carotenoids, bixin content and activity Antioxidant. The KS method provided flour with higher content of starch and amylose, but with a lower content of active compounds when compared to WS and AS flours. These flours were used to produce the films in different pH conditions (5, 7, 9) and heating time (1h, 2h, 3h, 4h) according to the complete 3x3x4 factorial design. According to the planning the best conditions were pH 5 and heating time of 2 hours for the KS flour films. KS flour films were produced in the best condition and characterized in their mechanical, functional and active properties, to be compared to the WS and AS flour films. KS flour films were more hydrophobic, more permeable to water vapor, more mechanically resistant, yet presented lower content of active compounds. The WS and AS flours films are less permeable to water vapor, moist, more soluble, more hydrophilic, more active and with a higher water absorption capacity when compared to KS flour films, but less mechanically resistant. The antimicrobial activity of aqua-alcoholic extract of urucum seeds and turmeric extract, as well as the WS, AS, KS urucum flour films were tested by the agar diffusion method against bacteria, fungi and yeast. Urucum and turmeric extracts presented antimicrobial efficiency against S. aureus, S. uberis and S. agalactiae, as well as C. albicans for turmeric. The urucum extract reduced the sporulation of the fungi A. fumigatus and Mucor sp., while the turmeric extract reduced the sporulation of the fungi A. tamarri, A. flavus, F. oxysporum and P. expansum. In the case of the films, only the film prepared with KS flour showed good activity against S. aureus, S. uberis, E. coli, P. aeruginosa, L. monocytogenes and C. albicans.

Desenvolvimento de filmes biodegradáveis a partir do resíduo da extração de corante de urucum