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MORELLI, V.G. In vitro evaluation of cobalt-chromium alloy disinfection with alkaline peroxide denture cleansers tablets. 2016. 110 P. Master ́s Degree Dissertation in Oral Rehabilitation – Faculdade de Odontologia de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto. The use of chemical methods for denture hygiene is essential to the longevity of prosthetic treatment. However, little has been reported about the antimicrobial action of effervescent tablets on metal structures of partial denture. The aim of this study was to evaluate the antimicrobial activity of 5 alkaline peroxide denture cleansers on disinfection of metal disks (12 mm x 3 mm) made of Cobalt-Chromium (Co-Cr) obtained from wax patterns made up in a metal matrix. There were obtained 252 Co-Cr alloy specimens (Degussa) polished in a standard way, randomly divided into 24 groups (n = 10) all of them were sterilized by ethylene oxide. Each experimental group, except CN, was inoculated with: Candida albicans (Ca), Candida glabrata (Cg), Streptococcus mutans (Sm) or Staphylococcus aureus (Sa). The adhesion of microorganisms occurred in 1 hour and 30 minutes in an incubator oven at 37°C. The biofilm growth occurred on 48 hours, with medium change every 24 hours, also at 37°C, while Sm process occurred in microaerophilic. Contaminated specimens were immersed for 5 minutes in those solutions: Polident 3 minutes® (P3), Polident for partials® (PP), Efferdent® (EF), Steradent® (ST) and Corega tabs® (CT) and sterile deionized water (positive control, PC). After cleansing the specimens were immersed in the Letheen broth culture medium and taken to the ultrasonic tank to release the still viable microorganisms. The obtained suspensions were diluted, 10, 10, 10 and 10, then seeded to determine the number of Colony Forming Units (CFU / mL). From the CFU values, were calculated values of Log10 for statistical analysis. Not-normal distribution of data to Ca, Cg and Sa was observed, it was used Kruskal-Wallis test (α = 0.05) followed by Dunn's posttest. Sm had normal data distribution and was used ANOVA test (α = 0.05) and Tukey posttest. Per the results there was no significant difference for Ca (p = 0.15). For Cg (p = 0.00) significant reduction of CFUs was observed with EF and CT in relation to PC. Sa (p = 0.006) had significant reduction of CFUs with ST when compared to CP. For Sm (p = 0.00) was observed significant reduction of CFUs with PP and ST in relation to PC. It was concluded that although some denture cleansers had antimicrobial activity against Cg, Sm and Sa, none was able to reduce the CFU number of Ca. Thus, the studied alkaline peroxides should be used with caution as disinfectant agents with Co-Cr structures, solely in 5 minutes of immersion, since they showed no antimicrobial action on all evaluated microorganisms.

Avaliação in vitro da desinfecção de liga de cobalto-cromo por meio de pastilhas higienizadoras efervescentes à base de peróxido alcalino