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MORAIS, R.C. In vitro study of the use of bioactive glass ceramic in affected dentin by natural and artificial caries: analysis of composites’s bond strength. Ribeirão Preto, 2014. 124p. Dissertation (Master’s Degree in Oral Rehabilitation). Ribeirão Preto School of Dentistry, University of São Paulo, Brazil. ABSTRACT The aim of this in vitro study was to evaluate, by means of microtensile test, the influence of bioactive glass ceramic’s application in the bond strength of adhesive systems with prior acid etching and natural or artificial caries-affected dentin compared to sound dentin. One hundred and twenty extracted human molars, 80 sound and 40 decayed, were selected, and 40 sound teeth were subjected to artificial caries formation by pH cycling. After occlusal surfaces’s regularization, teeth were divided into four groups (n = 10): Group 1 etching, rinsing and drying + Biosilicate + adhesive system; Group 2 same procedure as Group 1, without Biosilicate; Group 3 self-etching adhesive system + Biosilicate; Group 4 same procedure as Group 3, without Biosilicate. After all treatments, teeth were restored with composite (Opallis FGM). Then, teeth were stored in water at 37°C for six months and sectioned perpendicular to the bonding interface, producing sticks 0.9mm2 thickness. All samples were fixed into a testing machine (EMIC DL 2000 to 0.5 mm/min) and submitted to microtensile bond strength test. The fractures were observed in digital microscope (Keyence) and the fracture pattern was analyzed by scanning electron microscopy (JSM 5410, Sony). The tensile bond strength values (TBS) (2-way ANOVA, Bonferroni, p<.05) were: for sound teeth, G1 showed the best TBS values with statistical difference (p<.05) compared to the other groups, followed by G2 and G3, which showed no significant difference (p>.05) among themselves. The smaller TBS occurred for G4, with significant difference (p<.05) compared to the other groups. Following sound substrate, the second best TBS results occurred for teeth with artificial caries-dentin, at similar levels (p>.05) between all groups, except for G2. For the decayed teeth, there was no significant difference (p>.05) between groups. Groups in which Biosilicate was applied had a higher TBS (p <.05) when compared to untreated groups, which TBS was higher (p <.05) for the sound teeth group on which conventional adhesive system was used. There was no significant difference for the other substrates (p>.05). Regardless the substrate used, there were no significant differences (p>.05) in G4. The fracture pattern analysis showed predominance of adhesive fractures in all groups. It was concluded that the combination of conventional adhesive system and Biosilicate provided the greatest TBS between tooth and adhesive.

Estudo &lt;i&gt;in vitro &lt;/i&gt;do uso de vitrocerâmica bioativa em dentina afetada por cárie natural ou artificial: análise da resistência de união de compósitos