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YAMAMOTO, B. B. Analysis of the expression production and secretion profile of heat labile (LT) and heat stable (ST) toxins by enterotoxigenic Escherichia coli isolates. 2016. 62 f. Master thesis (Microbiology) – Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, 2016. Enterotoxigenic Escherichia coli (ETEC) is often associated with travelers’ diarrhea, and is a major cause of infant mortality in developing countries. To better colonize different niches, these bacteria need to adapt to different conditions imposed by the environment thus regulating the expression of their genes. ETEC adhere to the enterocytes in the gut by colonization factors (CFs) and causes watery diarrhea by secretion of heat-labile (LT) and heat-stable toxins (ST). There is no exclusive pattern of LT and ST secretion by ETEC isolates. Therefore, the aim of this study was to compare the secretion and production profile of LT and ST proteins, as well as to analyze the genes expression of these toxins in ETEC isolates. The results of this study showed that the eltA, sta1 and sta2 genes present in H10407 and 5 strains are differentially expressed when bacteria cell was cultivated in E. coli broth. Despite the parallel, there was no correlation between gene expression and secretion and production of the toxins, suggesting that there are other mechanisms involved in controlling the production of these toxins.

Análise do perfil de expressão, produção e secreção das toxinas termolábil (LT) e termoestável (ST) em isolados de &lt;i&gt;Escherichia coli &lt;/i&gt;enterotoxigênica.