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Emidio ECP. Melanin production by the dimorphic fungus Paracoccidioides lutzii and analysis of melanin as a virulence factor in the interface infection immune response. [dissertation (Master in Microbiology)]. São Paulo: Instituto de Ciências Biomédicas, Universidade de São Paulo; 2016. Paracoccidioidomycosis (PCM) is a granulomatous systemic mycosis, whose etiological agents are dimorphic fungi of the genus Paracoccidioides. Melanin production by various fungi interferes in the mechanism of pathogenesis, as in paracoccidioidomycosis. Thus, this project aimed to evaluate the production of melanin by isolates of P. lutzii and its in vitro effects by assessing the process of phagocytosis. The results obtained during this work showed that the isolates of P. lutzii (Pb01, Pb66, ED01, Pb1578 and Pb8334) produce melanin in different ways between them and also when compared with isolates of P. brasiliensis (Pb60855, Pb18 and Pbcão). Phagocytosis assays, were carried out with C57BL/6 mice peritoneal macrophages that were challenged with Pb18, Pb60855 and Pb01. Results showed that melanin reduced the percentage of phagocytosis of untreated yeast of Pb60855 and Pb18 and the opposite happened with Pb01. The analysis of the protein and enzymatic profiles, of the isolates Pb18, Pb60855 and Pb01, enabled to determine that the isolated Pb01 produces a smaller amount of proteins compared with Pb18 and Pb60855 in the tested conditions. The same observation was made in relation to the laccase enzyme activity.

Produção de melanina pelo fungo termodimórfico &lt;i&gt;Paracoccidioides lutzii&lt;/i&gt; e análise da melanina como fator de virulência sobre a interface infecção - resposta imune.