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AZEVEDO, D. S. Characterization of actin expression profile in Aedes aegypti. 2014. 89 p. Masters thesis (Biology of Pathogen Relationship -Host) Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, 2014. Understanding the basic molecular biology of insect vectors can contribute to the development of ways to combat the disease vector. In this context, our study was characterized to describe the expression profile of nine actin genes present in the genome of Aedes aegypti. Through sequences of complementary DNA (cDNA) available in databases, construction of specific primers for each gene actin was performed. First experiments of RT-PCR were made to obtain semi-quantitative transcriptional profiles of each actin gene using the primers constructed. We observed the constitutive expression of seven actin genes , two of which have been described in previous studies (AeAct AeAct-1 and-2) and the expression of stagespecific of two other genes, AeAct-3 for males and AeAct-4 for females, which also already described in the literature. After obtaining the semi-quantitative transcriptional profiles of all nine genes of actin, we also generate quantitative transcriptional profiles of those genes using qRT-PCR technique. The profiles obtained were similar to those reported in the literature for the first four actin genes. We observed the accumulation of the number of transcripts in all analyzed intervals for all genes, although there were intervals where these numbers come forward very low. The genes that presented with major peaks of induction were AeAct-4 and AeAct-3 and reported as expression of specific genes. And genes that showed minor peaks of induction were AeAct and AAEL005964-1. The peaks of induction for all genes always were found in immature mosquito, mainly in the pupa stage. This characteristic is possibly due to the fact that actin is a structural protein, justifying the high transcription in late larvae and mainly pupae stage where complete metamorphosis of insect occurs through a tissue restructuring. All transcriptional profiles obtained in this work shows us a broad overview of the behavior of these genes actin, although it takes us to many speculations about the function of each actin. This study describes the expression profile of five actin genes that have not yet reported in the literature.

Caracterização do perfil de expressão de actina em Aedes aegypti.