English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Tools annual

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Presents the access of premium content as premium feature

Premium Content

Global: Zendy Plus annual

Global: Zendy Free Plan

ISHIZUKA, E. K. Modulation of macrophage function by Nattectin: a C-type lectin from Thalassophryne nattereri fish venom. 2011. 78 p. Masters thesis (Immunology) Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, 2011. Nattectin is a 15 kDa toxin isolated from Thalassophryne nattereri fish venom that has homology with C-type lectin. The aim of this study was to evaluate the Nattectin ability to activate macrophage functions and the influence of Th1/Th2 cytokines in polarized activation of macrophages induced by Nattectin. Our results show that Nattectin was able to induce F4/80CD11bCD14Ly6C macrophage derived from bone marrow activation through CD40, CD80 and CD86 costimulatory molecules and MHC class II upregulation, and also an increase in integrins expression and endocytic capacity. It was found a positive dependence of Nattectin binding to glycosylated structures expressed in macrophages for induction of MHC class II, CD49a integrin expression and an increase in endocytic capacity. The expression of costimulatory molecules in macrophages was dependent the p38 MAPK signaling pathway and primarily PI3K signaling, while endocytic capacity and MMP-9 production was positively regulated through ERK1/2 signaling. We also demonstrated that phenotypic modifications in peritoneal and bone marrow-derived macropahges induced by Nattectin in C57BL/6 mice are consistent with classical (M1) activation of macrophages dependent on IL12 and IFN-γ cytokines, and negatively regulated by IL-4, IL-10 and IL-13. Finally, it was demonstrated an adjuvant role of IL-4 with IFN-γ in mobilization of SPMs (Small Peritoneal Macrophages) to peritoneal cavity and also in activation (expression of MHC class II and CCR7) of bone marrow-derived macrophages. Thus, Nattectin can be considered an important immunomodulatory agent able to modulate macrophage functions, useful in vaccines development and with therapeutic applications.

Modulação da função de macrófagos pela nattectina: uma lectina tipo C do veneno de Thalassophryne nattereri.