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OLIVEIRA, S. L. Study of genes differentially expressed in Aedes aegypti after infection with Plasmodium gallinaceum or Dengue viruses. 2011. XX f. Master thesis (Parasitology) – Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, 2011. Mosquitoes belonging to the family Culicidae (Diptera) play an important role as vectors of disease-causing pathogens that affect humans in various parts of the world (Marcondes, 2001), causing a great socioeconomic impact and public health areas in which they occur. Biological and behavioral aspects of mosquito also contribute decisively to its role as a transmitter of pathogens that affect human populations. The blood meal is a fundamental physiological process in the life cycle of these species. On the other hand, the infected blood meal causes changes in the metabolism of newly engorged females, triggering the differential expression of genes involved in immune responses, as well as genes involved in other physiological processes. As one of the interests of our group is to study the changes undergone by the insect vector after infection with the pathogen, in this work the starting point for this study was the selection of genes DV431431, DV340891 and DV411647 that the in silico analysis showed differential expression by Dengue virus infection of Plasmodium gallinaceum.Após or RT-PCR experiments in real time, it was shown that only the gene DV411647 had high expression 48 hours after the blood meal infected by both P. gallinaceum as for dengue virus. In the search for a predicted function of this gene in mosquitoes, we use the tool Editseq by DNAstar software to translate the peptide with the highest number of amino acids, and confronted by blastp with the GenBank database, we obtained a probable dehydrogenase / reductase short chain (AAK55494). Western blot experiments showed that this protein is present in the midgut of females 72 hours after feeding with blood infected with dengue virus type 2.

Estudo de genes diferencialmente expressos em Aedes aegypti após infecção com Plasmodium gallinaceum ou vírus de Dengue.