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63 Appendices 65 A.1 Script in R used for Primary Component Analyses (PCA) . . . . . . . . . . . . 66 A.2 DNA extraction protocol with Ammonium Acetate . . . . . . . . . . . . . . . 67 A.3 DNAdvance Extraction ProtocolTM . . . . . . . . . . . . . . . . . . . . . . . . 68 A.4 Purification Protocol with AMPURE . . . . . . . . . . . . . . . . . . . . . . . 69 A.5 Dasyatidae nucleotide sequences for MT-ATP6, MT-CYB and MT-ND2 for terminals used as outgroups . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70 A.6 Cestode nucleotide sequences acquired from previous studies . . . . . . . . . . 71 A.7 GenBank nucleotide sequences of cestodes for MT-RNR2 and LSU regions . . 89 A.8 Original hosts nucleotide sequences . . . . . . . . . . . . . . . . . . . . . . . 90 A.9 Original Acanthobothrium nucleotide sequences . . . . . . . . . . . . . . . . . 96

Acanthobothrium Blanchard, 1948 from the northwest Atlantic and their phylogenetic relationships with freshwater lineages