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71 Anexos 72 1 Material de hospedeiros disponiveis para o estudo . . . . . . . . . . . . . . . . 73 2 Material de cestoideos disponiveis para o estudo . . . . . . . . . . . . . . . . . 78 3 Medias das distâncias feneticas das matrizes de sequencias nucleotidicas de COI e nucleares . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89 4 Preparacao de lâminas de hologenoforos . . . . . . . . . . . . . . . . . . . . . 93 5 Protocolo otimizado para extracao de DNA genomico com DNAdvance TM . . 94 6 Protocolo otimizado para purificacao de DNA genomico com AMPure® kit . . 96 7 Hipotese Filogenetica utilizada no estudo . . . . . . . . . . . . . . . . . . . . 97 8 Arvore de consenso estrito da analise completa em TNT . . . . . . . . . . . . 98 9 Arvore de consenso estrito da analise parcial em TNT . . . . . . . . . . . . . . 99

Diversidade filogenética de Potamotrygonocestus Brooks & Thorson, 1976 (Eucestoda: Onchoproteocephalidea)