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Considering the need for green coconut water pasteurization after opening the fruit due to microbial and enzymatic (peroxidase (POD) and polyphenol oxidase (PFD)) action, the objective of this work is to study the behavior of the activity of these enzymes during continuous thermal processing. The first stage of the work was the study of the enzymes thermal inactivation kinetics during continuous pasteurization of coconut water, performed in a laboratory plate heat exchanger, Armfield 43 – A, at a temperature of 90 °C with different holding times. Samples of unprocessed and processed coconut water were frozen at 30 °C for enzymatic analyses. In the second stage, based on the results obtained, processing was performed at 90 °C with holding times of 80, 120, 180 and 200 s for studying the behavior of these enzymes during storage. Analyses of the pH, titratable acidity, turbidity and enzymatic activity were conducted during storage in processed and unprocessed samples kept at ambient temperature, refrigerated (10 °C) and frozen (20°C). Each coconut water batch was characterized by density, total and soluble solids, ashes, pH, titratable acidity and turbidity. For the continuous thermal processes carried out at 90 °C both POD and PFD presented at least two isozymes with different thermal stability, and PFD was more thermally resistant than POD. During storage at 30°C POD in processed coconut water was more stable than PFD. The highest physicochemical and enzymatic stability was achieved for processed coconut water under frozen storage.

Cinética de inativação térmica da peroxidase e da polifenoloxidase presentes na água de coco verde por processo térmico contínuo.