English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Tissue culture establishment of Sorghum bicolor Sorghum is a diploid monocot plant with a short life cycle of about 4 months. This crop is important in agriculture because it can be used for animal and human feed, and as well as source of raw material for biofuel production. Recently, sorghum also emerges as a strong candidate being a model plant on genomic and physiological studies related to cell wall in C4 grasses. Nevertheless, an efficient plant regeneration method is required for the establishment of genetic transformation technique to conduct the characterization of functional genes. In this context, the objective of this work was to establish a tissue culture protocol to evaluate the oxidation level of in vitro culture using leaf discs and immature embryos as explants, and testing different antioxidants and hormones concentrations for somatic embryogenesis and plant regeneration. In leaf discs, PVP, ascorbic acid and citric acid antioxidants were tested when manipulating the explants until their inoculation in culture media, but these factors failed to show any reduction in oxidation. In order to obtain somatic embryogenesis different concentrations of 2,4-D were tested for callus induction, 1,0 and 2,0 mg L -1 showed the best results. Several plant regeneration medium were tested however, only up to 1% of regeneration was achieved using leaf discs. In immature embryos, besides PVP and ascorbic acid antioxidants also asparagine and proline amino acids were tested in the callus induction media. The use of 2 g L -1 asparagine and 3 g L -1 proline in callus induction media and 3 mg L 1 TDZ in the regeneration media improved shoot regeneration efficiency up to 15%. Thus, the use of immature embryos was considered as a better option as explant for tissue culture of sorghum. Due to high production of oxidation and low frequency of plant regeneration in tissue culture, the genetic transformation via Agrobacterium tumefaciens known as “Floral Dip” method was tested. This technique does not require in vitro plant regeneration. Preliminary results were obtained, but the methodology needs to be improved.

Estabelecimento de protocolo de cultura de tecidos de Sorghum bicolor