English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Genetic transformation of oranges ‘Pera’ and ‘Natal’ (Citrus sinensis L. Osbeck) with the gene attacin A (attA), driven by preferentially expressed in phloem promoters for resistance to Candidatus Liberibacter spp. Currently, Brazil is a major citrus producer and the world’s largest producer of sweet oranges. However, diseases, such as huanglongbing (HLB) have seriously affected this production, causing significant losses in citrus production chain. HLB is associated with Gram-negative bacterias, restricted to the phloem of plants, called Candidatus Liberibacter spp., which besides reducing fruit production, can lead to plant death. An important strategy to control this disease is the production of transgenic plants expressing genes, specifically at the region of pathogen colonization. The aim of this study was to obtain transgenic plants of Citrus sinensis cv. 'Natal' and 'Pera', containing the gene attacin A (attA) that encodes an antibacterial peptide, driven by preferentially expressed in phloem promoters: AtSuc2 (sucrose transporter) or AtPP2 (phloem protein 2), cloned from Arabidopsis thaliana, or CsPP2 (phloem protein 2) cloned from Citrus sinensis. The identification of 65 transgenic plants was performed by PCR analysis. A lower number of transgenic events were verified using the gene construct pCAtSuc2/attA in relation events obtained with the gene constructs pCCsPP2/attA and pCAtPP2/attA. Plants identified as transgenic by PCR analysis were acclimatized and transferred to a greenhouse certified for growing transgenic plants. The Southern blot analyses were performed in acclimatized plants, which had sufficiently developed, confirming the integration of attA gene. The expression of attA gene was confirmed by RT-qPCR analysis. The transgenic plants obtained in this work, containing the gene attA directed by preferentially expressed in phloem promoters, will be further evaluated for resistance to Candidatus Liberibacter spp.

Transformação genética de laranjas 'Pera' e 'Natal' (Citrus sinensis L. Osbeck) com o gene atacina A (attA), dirigido por promotores preferencialmente expressos no floema, para resistência a Candidatus Liberibacter spp.